This work is focused on the development of miniaturized bio-analysis systems where cell lysing is needed to obtain intracellular materials for further analysis such as DNA and protein. This consists of a magnetically actuated micro-mixer for disrupting intracellular green fluorescent protein GFP), hydrophobic valve for cell lysate manipulation, and packed porous polymerized monolith chamber for solid phase extraction (SPE) and filtering debris of cell lysate. We adopt the method of using magnetically actuated force for lysing of GFP because it can greatly simplify purification steps for preparation of biological samples compared to conventional sample preparation by off-chip. The diaphragm of micro-mixer includes a NdFeB magnet, and it is actuated by a permanent magnet of a magnetic stirrer. The magnetically actuated micro-mixer induces reciprocating GFP sample and lysis buffer solution, and then well lysated sample with mixing time was introduced into the SPE chamber.

Schematic diagram of the magnetically actuated micro-mixer in the microdevice.