Suppressing Brownian motion of individual molecules in solution

PHYS 504

Adam Cohen, aecohen@stanford.edu, Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138 and W. E. Moerner, wmoerner@stanford.edu, Department of Chemistry, Stanford University, Stanford, CA 94305-5080.
The Anti-Brownian Electrokinetic Trap (ABEL trap) provides a means to trap and manipulate individual nanoscale objects in solution. The ABEL trap uses active electrokinetic feedback to cancel the Brownian motion of a single object of interest. We have trapped single molecules of DNA, proteins, viruses, lipid vesicles, and semiconductor nanocrystals. Due to its non-perturbative nature, the ABEL trap allows detailed studies of the conformational dynamics of single molecules of λ-DNA in their equilibrium, unstretched state. We identify the conformational normal modes, characterize their dynamics, and detect signs of nonlinear hydrodynamic coupling between modes--an effect present in the underlying equations of polymer dynamics but neglected in the Rouse and Zimm approximations. See: PNAS, 103, 4362-4365, 14 March 2006.
 

Poster Session
7:30 PM-10:00 PM, Wednesday, 13 September 2006 Moscone Center -- Hall D, Poster

Sci-Mix
8:00 PM-10:00 PM, Monday, 11 September 2006 Moscone Center -- Hall D, Sci-Mix

Division of Physical Chemistry

The 232nd ACS National Meeting, San Francisco, CA, September 10-14, 2006