ANYL 211 |
| The unique properties of nanomaterials offer excellent prospects for the detection of DNA and proteins. This presentation will describe new multi-amplification/multi-tag particle-based bioelectronic assays based on a variety new biomaterial-nanoparticle assemblies. In particular, combining the catalytic enlargement of the metal-particle tags with the effective ‘built-in' amplification of electrochemical stripping analysis, paved the way to remarkably low (fmol) detection limits. New amplification platforms for carrying numerous redox tags, including carbon nanotubes and polystyrene beads, will be discussed in connection to ultrasensitive detection of proteins and nucleic acids. The high sensitivity of the new protocols was combined with an efficient magnetic separation. Such use of magnetic beads has been extremely useful for discriminating against unwanted constituents, including a large excess of co-existing mismatched and non-complementary oligomers, chromosomal DNA, RNA and proteins. TEM imaging has indicated that the DNA hybrid links the metal nanoparticles to the magnetic beads. No such aggregates were observed in the presence of noncomplementary or mismatched DNA. A new electrochemical coding bioassay, based on different inorganic-colloid (quantum dots) nanocrystal tracers, whose metal components yield well-resolved highly sensitive stripping voltammetric signals for the corresponding targets, will be described. The use of DNA recognition for designing nanoscale assemblies with tailored properties will also be discussed. Current efforts aimed at developing and transforming these protocols to high throughput microchip and multiwell platforms will be discussed.
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Honoring Joe Wang, Recipient of the Electrochemical Analysis Award
8:30 AM-12:00 PM, Tuesday, 12 September 2006 Moscone Center -- Room 123, Oral
Division of Analytical Chemistry |