ANYL 214 |
| Nitrosothiols (RSNO), including nitrosoglutathione (GSNO) and nitrosocysteine (CysNO), exist in fresh blood and are formed from the reaction of corresponding thiols with oxidation intermediates of nitric oxide (NO) produced by endothelial cells (EC). Such RSNO species are thought to serve as stable carriers of NO in the bloodstream. RSNOs can also serve as substrates to generate NO at the surface of thromboresistant catalytic polymers and at the surface of platelets. Detection of RSNO levels in blood may serve as an indicator of NO production by the EC, and thereby be predictive of risk for thrombotic events. In this presentation, new RSNO sensors will be described based on use of Cu(II) or organoselenium catalytic species as well as certain enzymes immobilized at the surface of either electrochemical NO detectors or chemical reactors combined with chemiluminescence NO detection. It will be shown that reversible sensors for monitoring RSNO levels can be prepared with detection limits in the 1-1000 nM range.
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Honoring Joe Wang, Recipient of the Electrochemical Analysis Award
8:30 AM-12:00 PM, Tuesday, 12 September 2006 Moscone Center -- Room 123, Oral
Division of Analytical Chemistry |