ANYL 151 |
| Many naturally occurring pyrrolizidine alkaloids (PAs) are hepatotoxic, which may cause liver damage and in some cases liver cancer. Toxic PAs are often responsible for serious health problems through direct consumption of PA-containing herbal teas, herbal medicines, and herbal dietary supplements. In this study, methods for the extraction and analysis of toxic pyrrolizidine alkaloids were developed. In particular, the combination of microwave extraction with a novel on-column electrokinetic focusing method based on hyphenation of dynamic junction and sweeping techniques for enhancement of sensitivity in the analysis of these alkaloids by capillary electrophoresis was investigated. Optimization of the conditions for microwave extraction, as well as for on-column electrokinetic focusing was performed systematically. Critical factors such as microwave power, irradiation/extraction time, extraction solvent, sample matrix, pH, salt concentration, etc were studied. Subsequently, the optimized conditions were used for the extraction and analysis of toxic alkaloids in commercially available herbal products. |
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General Papers
7:00 PM-9:00 PM, Sunday, 10 September 2006 Moscone Center -- Hall D, Poster
Division of Analytical Chemistry |