ANYL 161 |
| It has long been known that DNA microarray plays a pivotal role in genomic study and diagnostics, where massively parallel analyzing nucleic acid becomes routine using this new concept of microarrays. Beyond all doubts, DNA microarray technique has had an explosive influence on new biological information, human genotyping, gene related drug metabolism. The past decade also has been witnessed this research tool applied increasingly to questions of clinical and medical relevance. Therefore, many manufactories focused on fabrication of DNA microarrays and supplied a number of commercial products, such as Affymetrix and Nimblegen. In terms of the property of arrayed DNA probe with known identity, there are two variants of DNA array technology. Traditionally, the probe is immobilized on a solid support using robot spotting, including pipette, drop-touch, piezoelectric, ink-jet, etc. In another format, an array of oligonucleotides is synthesized in situ (on-chip) using photolithography or electrochemistry. However, most DNA chips employed glass as a solid substrate, both because of cheap resource and easy preparation. The downscale of glassy materials is obviously unstable toward biomolecule immobilization and less chemical uniform, which seriously limits DNA chip within once-use and short storage period. Diamond materials as an alternative substrate might dramatically improve stability of DNA chip and open the new route for multiple usable microarrays. In this project, both of DNA format techniques have been utilized in array fabrication. DNA sequences can be either spotted or synthesized in situ. Stability of this new substrate has been studied by multiple cycles of hybridization-dehybridization and long period (a year) storage in dry environment at room temperature. We clearly demonstrated the diamond as a stable solid support in DNA microarray. The capability of reuse and multiple-time measurements of diamond microarrays might allow extensive application of this technique in numbers research fields |
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General Papers
7:00 PM-9:00 PM, Sunday, 10 September 2006 Moscone Center -- Hall D, Poster
Division of Analytical Chemistry |