ANYL 306 |
| Polyacrylamide has been used for capillary wall coating for decades. This coating protocol was first introduced by Hjerten in 1985. Typically, the silanol groups on a capillary wall are first covalently bonded to a bi-function reagent containing vinyl groups, and these vinyl groups are then polymerized with acrylamide, forming a layer of linear polyacrylamide (LPA). The major problem of this coating is that the LPA molecules cannot cover the capillary wall completely. The poorly covered area will adsorb proteins and create EOF. In this report, we present a cross-linked polyacrylamide (CPA) coating and demonstrate the performance of this coating for high quality CIEF, CGE and CZE separations of complex protein samples. Using these CPA-coated capillaries, the electroosmotic mobility is reduced to 1.0x10-10 m2 V-1 s-1. More than 100 proteins are resolved in both CIEF and CGE separations. More than two million plates are routinely obtained in CZE separations. |
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Analytical Approaches: Separation Science
8:30 AM-12:00 PM, Thursday, 14 September 2006 Moscone Center -- Room 130, Oral
Division of Analytical Chemistry |