ANYL 4 |
| When biomembranes are subjected to forces present in typical analytical techniques, many complex structures and behaviors can result. One such case is demonstrated for a biological mimic, liposomes, in the presence of an electric field at a typical strength used in capillary zone electrophoresis. In this particular case, these structures and behaviors both complicate data interpretation and provide unique opportunities. Historical theoretical treatment for the migration of these bioparticals assumes that they are hard spheres with uniform charge, however, liposomes and biomembranes are quite soft and have mobile charge about the surface. These properties greatly influence the analytical analysis of liposomes and provide unique structures. The unique structures are for both the individual liposome and for the network of rheologic patterns during migration. In this presentation I will present some seemingly confounding results on the migration of liposomes under capillary electrophoresis conditions, compare these results with shape and multipole models of migration which take into account the properties of liposomes. Further, we have imaged the liposomes by scanning electron microscropy and video fluorescence microscopy and have found many un-expected structures both at the nanoscopic scale and at the 10s of micro level. We show several millimeter long membrane-bound nanotubules formed from liposomes by application of an electric field. |
|
Honoring Andrew Ewing, Recipient of the Chemical Instrumentation Award
8:30 AM-10:50 AM, Sunday, 10 September 2006 Moscone Center -- Room 123, Oral
Division of Analytical Chemistry |