ANYL 277 |
| Two-dimensional chromatography has undergone explosive growth over the past decade and High Performance Liquid Chromatography (2D-HPLC) in particular has attracted tremendous attention especially from bio-scientists due to its potential for significantly increased peak capacity compared to conventional 1D-HPLC, and its ability to handle the extremely complex samples encountered in the biological sciences. This is exemplified by the use of 2D-HPLC in conjunction with mass spectrometry which has become a major working tool in proteomic studies. Unfortunately relatively little work has been done to develop general guidelines for the practical optimization of the technique in terms of peak capacity and peak capacity production. We will present a critical comparison of 1D- and 2D-HPLC from the perspective of peak capacity and peak capacity production, and discuss factors influencing the distribution of peak capacity between the two dimensions of a 2D-HPLC system. Fast 2D-HPLC separations of plant extracts with peak capacities exceeding 1000 and run times less than one hour will be presented to show the advantages of doing ultra-fast high-temperature gradient elution LC in the second dimension of the 2D-HPLC system. |
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Analytical Approaches: Separation Science
1:30 PM-5:00 PM, Wednesday, 13 September 2006 Moscone Center -- Room 130, Oral
Division of Analytical Chemistry |