ANYL 124 |
| The Asher group has recently developed Polymerized Crystalline Colloidal Array (PCCA) sensor materials that can monitor glucose concentration. We used polyacrylamide-based PCCA sensors with boronic acid functional groups to monitor glucose in aqueous solutions. This work suggests the possibility of using PCCA sensors to non-invasively monitor glucose in tear fluid. While numerous studies have attempted to measure glucose concentrations in tear fluid, there is substantial disagreement among them. In addition, there is little information on the time correlation between blood and tear glucose levels. Tear and blood samples were collected at standard intervals from fasting patients and from patients fed a standardized meal. The tears were collected with micro capillaries using a slit lamp microscope to avoid stimulating lacrimation. Tear glucose was quantitated using Electrospray Ionization Mass Spectrometry with isotopic glucose as an internal standard. Preliminary results show that a normal fasting tear glucose concentration is below 50 ìM, and about four-fold lower than previously reported. Increased tear glucose concentrations were observed in some subjects wearing contact lenses. Though there is significant variation between individuals, a correlation between blood and tear glucose levels is evident in individuals consuming a standardized meal. Tear glucose levels also varied between the two eyes of a single subject. The present study provides useful information for developing a non-invasive glucose sensor that monitors tear glucose levels. Despite some variation between individuals, tear glucose concentration can serve as an indicator of blood concentration. This work also lays a foundation for the non-invasive monitoring of many natural and pharmacological metabolites.
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General Papers
7:00 PM-9:00 PM, Sunday, 10 September 2006 Moscone Center -- Hall D, Poster
Division of Analytical Chemistry |