ANYL 160 |
| Specific antibody against acrylamide, an ultra small molecule with toxicity and potential carcinogenicity, was produced for the first time and subsequently used to establish a competitive biotin-avidin amplified enzyme-linked immunosorbent assay (BA-ELISA) to detect acrylamide in foods. The affinity constant of the antibody-antigen was found to be 2.44°Ñ106 L/mol based on SPR measurements and little cross-reactivity was observed to most of the tested structurally related substances. The results demonstrated the polyclonal antibody to be highly affinitive and specific to the conjugate structure with an ethylenic bond and a carbonyl group in acylamide molecule. After an optimized sample preparation, far simpler than those required by most existing methods, food samples can be successfully analyzed by the established BA-ELISA. The concentration of acrylamide in the tested fried potatoes was found to be 7.9„b0.3 mg/kg with recoveries of 88-110% based on the BA-ELISA, which was in good agreement with the results obtained using high-performance liquid chromatography (HPLC) as a reference method. The BA-ELISA offers a rapid, simple, cost-effective and high-throughput alternative for acrylamide quantification in food and casts light upon development of more immunochemical analytical methods for other ultra small molecules. Acknowledgements The work was supported by the National Natural Science Foundation of China (Grants No. 20305002). References (1) Mottram, D. S.; Wedzicha, B. L.; Dodson, A. T. Nature 2002, 419, 448 - 449. (2) Detection and Quantitation of Acrylamide in Food. http:°üwww.cfsan.fda. gov/-dms/acrylami.html. |
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General Papers
7:00 PM-9:00 PM, Sunday, 10 September 2006 Moscone Center -- Hall D, Poster
Division of Analytical Chemistry |