ANYL 244 |
| The objective of HPLC method development is the "identification of chromatographic conditions which meet the goals of the analysis". In an industrial setting, this objective must be modified to include the phrase "as quickly and as efficiently as possible". Emphasizing reversed-phase chromatography, this presentation will explore the parameters (retention, selectivity, and efficiency) and the variables (column dimensions, particle size, flow rate, temperature, mobile phase strength, organic solvent type, column type, pH, and additive type/concentration) which must be manipulated in order to attain this objective. We will focus on identifying and ranking the key variables and outline strategies for systematically exploring their effects which maximizes the probability of meeting analytical goals while minimizing experimental time and effort. |
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Methods Development for Pharmaceutical Analysis
1:30 PM-5:00 PM, Tuesday, 12 September 2006 Moscone Center -- Room 124, Oral
Division of Analytical Chemistry |