BIOL 160 |
| Abstract: The primary structure of O-linked glycan chain of tea glycoprotein(TGP), that was purified from coarse old green tea picked from Fenyi County of Jiangxi Province of China by using the ÄKTA purifier100 biological macromolecule purified system, was determined by using many chemical methods (sugar component analysis, uronic component analysis, HIO4 Oxidation Reaction, Smith Degradation, methylation analysis, partial acid hydrolysis, etc.) and many instrumental methods (such as UV, IR, NMR, GC-MS etc.). The results showed that the O-linked glycan chain consists of 7 monosaccharides: Rha, Ara, Xyl, Glc, Man, Gal and GalA with molar ratios of 4.19:3.98:1.84:1.00:5.81:8.35:1.51. The primary structure of the O-linked glycan chain was outlined as below: the core structure of the main chain consists of GalA, Rha, Glc and Gal units and their molar ratio are 1:4:6:8 and Gal exists at the terminal end. The branch chains maybe exist at C-4 of Rha, C-3 of Glc, Gal and GalA. The branch chain consists of Ara, Xyl and Man, and Ara exists at the terminal end of the branch chains. All monosaccharide linkages are β- forms. Key words: Tea glycoprotein, The O-linked glycan chain, Primary structure, HIO4 Oxidation Reaction, Smith Degradation, methylation analysis, GC-MS, NMR, IR, UV * Supported financially by “The National Natural Science Foundation of China (No: 20462005).” **Corresponding author: Minyong Xie, Ph.D in Nutrition, Professor in Food Science, Tel.: +86/791/3969009, Email: myxie@ncu.edu.cn
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Chemistry and Metabolism
4:30 PM-6:30 PM, Tuesday, 12 September 2006 Moscone Center -- Hall D, Poster
Division of Biological Chemistry |