Protein labeling in live cells

BIOL 150

Youn-Hi Woo, woo20@llnl.gov, Chemicall Biology and Nuclear Sciences Division, Lawrence Livermore National Laboratory, 7000 East Ave, Livermore, CA 94550, Julio A Camarero, camarero1@llnl.gov, Chemical Biology & Nuclear Science Division, Lawrence Livermore National Laboratory, 7000 East Avenue, L-231, Livermore, CA 94550, and Lisa Stubbs, stubbs5@llnl.gov, Bio Science Directorate, Lawrence Livermore National Laboratory, 7000 East Ave, livermore, 94550.
Characterization of proteins in living cells is required to accurately understand their functions, localization and other properties. Here we present a traceless and efficient way of labeling proteins in vivo with fluorescent probes, utilizing intein chemistry and a protein transduction peptide.
 

Chemistry and Metabolism
4:30 PM-6:30 PM, Tuesday, 12 September 2006 Moscone Center -- Hall D, Poster

Division of Biological Chemistry

The 232nd ACS National Meeting, San Francisco, CA, September 10-14, 2006