BIOL 119 |
| We have examined the structure of telomeric sequence d(T2AG3)4 (H24) in potassium solution by using a 3,6-bis(1-methyl-4-vinylpyridinium)carbazole diiodide (BMVC) molecule. A rapid conversion of the circular dichroism (CD) pattern of H24 after adding 50 mM of potassium into 150 mM of sodium solution led us to suspect the presence of the propeller G-quadruplex (G4) structure and the (3+1) model for G4 structure of H24 in potassium solution. Particularly, no appreciable change on the induced CD spectra of BMVC is found during the change of the CD patterns of H24 upon potassium titration. Further study in substituting the base in the loops suggested that both conformations are unlikely the major structures of H24 in potassium solution. It is likely that the structure of H24 in potassium solution is similar to that in sodium solution. Different CD patterns are probably due to loop interaction resulting from different sizes of sodium and potassium. |
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Chemistry and Metabolism
4:30 PM-6:30 PM, Tuesday, 12 September 2006 Moscone Center -- Hall D, Poster
Division of Biological Chemistry |