Bioactivity driven fractionation of pomegranate extract and characterization of its phenolic composition by mass spectometry

CELL 13

Erika Salas, esalas@ansci.wisc.edu1, Linda G. Haas2, Brian B. Carey2, Christian G. Krueger, ckrueger@wisc.edu3, Martha M. Vestling, vestling@chem.wisc.edu4, and Jess D. Reed, jdreed@wisc.edu3. (1) Facultad de Ciencias Quimicas y Tecnologia de Alimentos, Universidad Autonoma de Chihuahua, ciudad universitaria s/n, Chihuahua, 31170, Mexico, (2) Department of Animal Sciences, University of Wisconsin-Madison, 1675 Observatory Drive, madison, WI 53706, (3) Department of Animal Sciences, University of Wisconsin-Madison, 1675 Observatory Drive, Madison, WI 53706, (4) Department of Chemistry, University of Wisconsin
The health benefits of pomegranate polyphenols have focused on a few individual compounds (punicalagin, ellagic acid). However, the structural diversity of polyphenolics in pomegranates is much greater than is currently appreciated. Commercial pomegranate extract, was fractionated by low pressure chromatography on Sephadex LH-20. Five different fractions were obtained by sequential elution with aqueous/organic solvents, tested for anti-inflammatory bioactivity and then analyzed by HPLC/DAD/ESI-MS and MALDI-TOF MS. Preliminary results showed that the first two fractions showed no bioactivity, the third (containing punicalin) and forth (containing ellagic acid) fractions showed some bioactivity at the highest dose, the fifth fraction (containing punicalagin and gallagic acid) dose dependently inhibited COX-2 expression in bacterial lipopolysaccharide stimulated macrophages. This fraction was further fractionated to separate punicalagin from the rest of the monomeric and oligomeric gallotannins and ellagitannin and each subfraction was tested for bioactivity and characterized by mass spectrometry.
 

Poster Session
4:00 PM-6:00 PM, Sunday, 10 September 2006 Moscone Center -- Hall D, Poster

Division of Cellulose & Renewable Materials

The 232nd ACS National Meeting, San Francisco, CA, September 10-14, 2006