Detailed phospholipid follow-up during atherogenic modifications of low density lipoprotein particles by 1H NMR spectroscopy at 800 MHz

BIOL 107

Pasi PK. Soininen, pasi.soininen@uku.fi1, Reino Laatikainen1, Katariina Öörni2, Hannu Maaheimo3, Petri T Kovanen2, Kimmo Kaski4, and Mika Ala-Korpela4. (1) Department of Chemistry, University of Kuopio, P.O.Box 1627, Kuopio, 70211, Finland, (2) Wihuri Research Institute, Kalliolinnantie 4, Helsinki, 00140, Finland, (3) NMR-laboratory and Structural Biology and Biophysics Program, VTT Biotechnology, Helsinki, Finland, (4) Laboratory of Computational Engineering, Systems Biology and Bioinformation Technology, Helsinki University of Technology, Helsinki, Finland
Cholesterol in atherosclerotic lesions originates mostly from lipid droplets formed from modified low-density lipoprotein (LDL) particles. Understanding of these modifications calls for dynamic physicochemical studies on LDL modifications. There is a lack of techniques that would facilitate a non-destructive and dynamic follow-up of molecular processes in a native LDL sample under an enzymatic attack in a physiological environment. However, 1H NMR seems a feasible choice. Particularly, since our recent studies on the effects of phospholipase A2 (PLA2) on LDL structure at 800 MHz, for the first time, distinguish all major phospholipids, including lysophosphatidylcholine, at the LDL particles. The novel opportunity to quantify lysophosphatidylcholine allowed us to study the effects of oxidation on the LDL-bound PLA2. Our preliminary data indicate activation of the LDL-bound PLA2 at the very early stages of peroxidation.

 

Chemistry and Metabolism
4:30 PM-6:30 PM, Tuesday, 12 September 2006 Moscone Center -- Hall D, Poster

Division of Biological Chemistry

The 232nd ACS National Meeting, San Francisco, CA, September 10-14, 2006