Electrochemical-surface plasmon resonance (SPR) glucose biosensors based on conducting polymer/glucose oxidase multilayer films

POLY 24

Rigoberto C. Advincula, radvincula@uh.edu, Departments of Chemistry and Chemical Engineering, University of Houston, 4800 Calhoun Road, 136 Fleming Building, Houston, TX 77204-5003, Akira Baba, ababa@uh.edu, Department of Chemistry, University of Houston, 136 Fleming Building, Houston, TX 77204-5003, and Wolfgang Knoll, knoll@mpip-mainz.mpg.de, Max-Planck-Institut für Polymerforschung, Postfach 3148, 55021 Mainz, Germany.
Conducting polymers are interesting materials for monitoring and manipulate enzyme interactions. This is attributed to the high conductivity and specific redox properties of conducting polymers which is essential for redox enzyme communications. The surface plasmon resonance (SPR) optical technique is a powerful tool for the characterization of surfaces, interfaces, and thin films. In bio-sensor applications, this technique allows for the investigation of biomolecular adsorption/desorption events onto surfaces and provides in-situ time dependent surface coverage measurements without the need for labels. In this work, we extensively apply the electrochemical (EC)-SPR techniques for the development of highly sensitive bio-sensors and novel biological opto-electronic applications. In order to achieve a controlled enzyme electrode, GOx (a negatively charged material) was immobilized with positively charged conducting polymers using the layer-by-layer (LBL) self-assembly fabrication technique.
 

Polymers in Biosensors and Biochips
8:30 AM-12:10 PM, Sunday, 10 September 2006 San Francisco Marriott -- Salon 12/13, Oral

Division of Polymer Chemistry

The 232nd ACS National Meeting, San Francisco, CA, September 10-14, 2006