Performance validation of the flow through rapid test and microtitre plate enzyme immunoassay test for ochratoxin A in wine using a quantitative HPLC method

AGRO 157

L. Sibanda, sibanda@toxi-test.be1, S. De Saeger2, R. Verheijen3, C. Arts3, E. Grutters3, L. Streppel3, and Carlos Van Peteghem4. (1) TOXI-TEST N. V, Harelbekestraat 72, Gent, B-9000, Belgium, (2) Laboratory of Food Analysis, Ghent University, Ghent, Belgium, (3) Euro-Diagnostica B. C, Beijerinckweg 18, Arnhem, Netherlands, (4) Department of Bioanalysis, Ghent University, Harelbekestraat 72, Ghent, 9000, Belgium
The Flow Through Rapid Test (FTRT) kit and the microtitre plate Enzyme Immunoassay (EIA) from Euro-Diagnostica BV were successfully validated at the Laboratory of Food Analysis, Ghent University, in Belgium. The FTRT and EIA test kits were used for screening red wine for ochratoxin A (OTA); the results were validated by HPLC analysis. The samples which consisted of red and rose wines were from diverse origins. The FTRT test kit contained everything required for the test. The FTRT and EIA were performed according to the manufacturer's instructions. The EIA had an average recovery of 72%. According to the FTRT screening results, 10 samples (26%) of the 38 red wine samples were positive for OTA. The HPLC confirmation showed that 21 samples (55%) were contaminated with OTA and 6 samples (16%) had levels above the 1 ppb cut-off level. Four samples (10%) gave a false positive result and there were, however, no false negative results. The Flow Through Rapid Test, therefore, had a 90% correlation with the HPLC confirmatory analysis.
 

Recent Advances in Immunochemistry and their Applications to Agrochemicals Poster Session
1:00 PM-5:00 PM, Tuesday, 12 September 2006 Moscone Center -- Hall D, Poster

Division of Agrochemicals

The 232nd ACS National Meeting, San Francisco, CA, September 10-14, 2006