POLY 255 |
| There is considerable interest in identifying the simuli that elevate endogenous synthesis of the mediaotrs and much research has been conducted to treat the inflammation in the body by directly introducing the anti-inflammatory cytokines via either intra-injection or oral administration. The objective of this research is to develop an anti-inflammatory and immune-suppressive cytokine immobilized non-fouling surface that eliminates all adventitious adsorption of protein in a robust manner and that optimally leads specific anti-inflammatory interaction with immune cells around the implants for both attenuating inflammation and promoting wound healing response. For temporally profiling the host of cytokines secreted by the immune cells under biomaterials stimulation, a new tool is required that can describe the state of many cytokines simultaneously at low cost, with minimal sample volumes. We have been working on high throughput microarray technology to measure this cell response both in vivo and in vitro. We have successfully developed an IL-1ra immobilized non-fouling surface using SAMs and the immobilized IL-1ra showed that it maintain it's bioactivity after immobilized until 72hrs. The immobilized IL-1ra on SAM surface reduced the pro-inflammatory cytokine expression indicating attenuating inflammation. |
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Polymers in Biosensors and Biochips
8:30 AM-11:50 AM, Monday, 11 September 2006 San Francisco Marriott -- Salon 12/13, Oral
Division of Polymer Chemistry |