Cell based bioassays in microfluidic systems

POLY 73

Michael V. Pishko, mpishko@engr.psu.edu, Departments of Chemical Engineering, Chemistry, and Materials Science and Engineering, The Pennsylvania State University, 204 Fenske Lab, Shortlidge Road, University Park, PA 16802
Here we will discuss the development of cell bioassay systems based mammalian cell arrays of differing phenotype integrated with microfluidics and microsensors used to monitor cellular effects of multiple chemical and biological candidates. To fabricate these arrays, we immobilize either single or small groups of cells in 3-dimensional hydrogel microstructures fabricated on plastic or glass surfaces. The specific and non-specific response of these cells to target molecules is monitored using optical detectors and analyzed to quantify the effect of these agents on the different phenotypes present in the array. Assays performed in these systems include for example, viability, apoptosis, NO production, and cell-cell interactions. Such arrays may be used in high content, high content drug candidate screening or pathogen detection for food safety.
 

Polymers in Biosensors and Biochips
1:30 PM-4:40 PM, Sunday, 10 September 2006 San Francisco Marriott -- Salon 12/13, Oral

Division of Polymer Chemistry

The 232nd ACS National Meeting, San Francisco, CA, September 10-14, 2006