AGRO 155 |
| Immunoassays are used as screening tools for human exposure assessment to a variety of agrochemicals. Recently, efforts to improve immunosensing by miniaturization and introducing novel fluorescent nanostructures have gained attention. Quantum dots (QDs) are a relatively new class of fluorophores with high quantum yield, excellent photostability, and narrow photoluminescence emission. Here we present the application of QDs as labels in immunoassay microarrays for the multiplex detection of 3-phenoxybenzoic acid (PBA) and atrazine-mercapturate (AM). PBA is a biomarker of exposure to the highly potent pyrethroid insecticides and AM is a biomarker for exposure to the herbicide atrazine. Microarrays were fabricated by microcontact printing of the coating antigens in line patterns onto glass substrates. Competitive immunoassays were successfully performed using quantum dots (QD560 and QD620) as reporters. The multiplexed immunoassays were characterized by fluorescence microscopy and SEM. The application of QD fluorophores facilitates multiplex assays and therefore can contribute to enhanced throughput in biomonitoring. |
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Recent Advances in Immunochemistry and their Applications to Agrochemicals Poster Session
1:00 PM-5:00 PM, Tuesday, 12 September 2006 Moscone Center -- Hall D, Poster
Sci-Mix
Division of Agrochemicals |