Parallel synthesis and high-throughput solubility determination of NCGC produced libraries:  Models for probe development

            In addition to activity and potency, one major requirement for probe development at the NIH Chemical Genomics Center is solubility.  The ability to determine solubility of compound libraries in a high-throughput fashion is very important.  One approach, using the Veloce Nanostreem parallel HPLC system and analysis software, has been tested on a purine library synthesized at the NCGC.  Synthesis (Scheme 1), results and applicability to a high-throughput environment are reported, along with follow-up experiments.