ANYL 259 |
| Microchip electrophoresis is becoming increasingly popular due to the many advantages inherent to the miniaturized planar format. These include the ability to analyze small sample volumes and employ high separation field strengths, as well as the possibility to integrate sample preparation steps. In this paper, the on-line coupling of microdialysis to microchip electrophoresis for continuous near real time monitoring of peptides and neurotransmitters is described. Two different interfaces have been developed to allow for the injection of discrete samples from the microdialysis flowing stream. The first uses an electrokinetically actuated flow gated interface to move the sample plug into the separation channel. The second approach employs a PDMS valve. The advantages and disadvantages of the two approaches will be discussed along with application of the devices for the near real time monitoring of biological substances in vitro and in vivo.
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Interfacing Biology with Lab-On-A-Chip Separations
8:30 AM-11:55 AM, Wednesday, 13 September 2006 Moscone Center -- Room 130, Oral
Division of Analytical Chemistry |