CARB 63 |
| It must be emphasized that the difficulty of the glycomics/glycoproteomics study strongly depends on the tedious and time-consuming procedures for the purification of faint amount of oligosaccharides and glycopeptides from biological materials. We communicated that rapid and efficient purification of carbohydrates can be achieved by employing sugar-specific chemical ligation with aminooxy-functionalized polymers, which we termed “glycoblotting”. The chemoselective blotting of oligosaccharides onto synthetic polymers relies on the unique oxime bond formation between aminooxy group and aldehyde/ketone group at the reducing terminal of oligosaccharides, thus enabling highly selective and rapid oligosaccharide purification. We found captured oligosaccharides are recovered as newly formed oxime derivatives of the O-substituted aminooxy compound added, thus enabling concomitant releasing and probing. Our glycomic technique can be implemented in existing biochemistry and molecular diagnostics laboratories because enriched sugars and glycopeptides by solid phase trans-oximization with high-sensitive labeling reagents are widely usable in a variety of common analytical methods. |
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General Posters
8:00 PM-10:00 PM, Tuesday, 12 September 2006 Moscone Center -- Hall D, Poster
Division of Carbohydrate Chemistry |