CARB 47 |
| Ricin is a highly toxic 65 KDa glycoprotein isolated from the seeds of Ricinis communis. The easy availability and high toxicity makes it a potential toxin warfare agent. Ricin toxin possesses two chains, A and B, linked by a disulfide bond. The B chain is responsible for binding events and galactose is the preferred sugar for binding. The B chain binds to galactose residues present on the surfaces of cells, and enables the cytotoxic A chain to gain access into the cell and destroy it. Single crystal X-ray structural studies have revealed the existence of multiple binding sites in the B chain, as have biochemical and mutational analysis. Therefore, tethered galactose residues at the termini of a multidentate core should provide a high affinity ligand for Ricin binding. The synthesis of a rigid scaffold and attachment of galactose derivatives to the rigid scaffold via flexible oligoethylene glycol linkers will be presented. In addition, binding studies using ITC will be presented. |
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General Posters
8:00 PM-10:00 PM, Tuesday, 12 September 2006 Moscone Center -- Hall D, Poster
Sci-Mix
Division of Carbohydrate Chemistry |