CARB 79 |
| We investigated the fragmentation behavior of the protonated glycopeptides and sodiated oligosaccharides using infrared multiple photodissociation (IRMPD) with a tunable IR free electron laser (FEL) in conjunction with Fourier-Transform Ion Cyclotron Resonance (FTICR) mass spectrometry (MS). The fragmentation behavior of these parent ions was probed by monitoring the fragmentation of the ions induced by IRMPD in the IR wavelength range of 5.7–9.5 µm. The gas-phase IRMPD spectroscopy was obtained for the large gas phase molecular ions. The results show that an abundance of the product ions from the peptides was mainly observed at 6.1 µm, corresponding to the amide I band; these predominated due to contributions from the C = O stretching vibration of the peptide backbone. For the oligosaccharides, the intensity of the fragment ions gradually increased on increasing the wavelength from 8.5 µm. The IR spectra peaked at a wavelength of around 9.1 µm. |
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Chemical Glycobiology Symposium
7:00 PM-9:00 PM, Tuesday, 12 September 2006 Moscone Center -- Hall D, Poster
Division of Carbohydrate Chemistry |