Peptide-modified responsive surfaces with built-in logic for sensing and signal transduction

POLY 253

Ryan Toomey, rtoomey@eng.usf.edu1, Peng Tian2, and Lawrence Neuman2. (1) Department of Chemical Engineering, University of South Florida, 4202 E. Fowler Ave ENB 118, Tampa, FL 33620, (2) Department of Chemical Engineering, University of South Florida
The overall thrust of our research program is to develop responsive surfaces without the need for complex circuitry. The approach is to use surface-attached, cross-linked polymer films that can be toggled from a collapsed to a swollen state or vice-versa in response to an external target. The target alters the balance of hydration forces in the responsive layer, resulting in changes to the swelling of the layer. To this end, we demonstrate that the transition temperature of poly-N-isopropylacrylamide (Poly-NIPAAm) layers can be governed by the conformation of embedded peptide sequences. The sequences are grown directly in a poly-NIPAAm network copolymerized with an amine terminated monomer, N-(3-aminopropyl)methacrylamide hydrochloride, using standard Merrifield synthesis techniques. We can build logic directly into the layer structure via the conjugated peptides, which alter the response characteristics of the layer depending on the conformation of the peptide.
 

Polymers in Biosensors and Biochips
8:30 AM-11:50 AM, Monday, 11 September 2006 San Francisco Marriott -- Salon 12/13, Oral

Division of Polymer Chemistry

The 232nd ACS National Meeting, San Francisco, CA, September 10-14, 2006