Synthesis of polymer nanoparticles from amphiphilc block copolymers for DNA detection via amplified fluorescence

POLY 471

Brian R. Stepp, b-stepp@northwestern.edu1, Megan Boyle, mboyle01@saintmarys.edu2, Sharan Srinivasan1, and SonBinh T. Nguyen, stn@northwestern.edu1. (1) Department of Chemistry, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208-3113, (2) Department of Chemistry, Saint Mary's College, Saint Mary's College, Notre Dame, IN 46556
Amphiphilic block copolymers containing pendant terthiophene and tosylated-poly(ethylene glycol) groups have been synthesized, and were subsequently used in the formation of core-shell polymer nanoparticles (PNP's). The tosylate functionality is expressed on the periphery of the particles and is reactive to amine terminated oligonucleotides. The above properties will facilitate DNA-modification of the PNP's and allow them to function as the probe in a three-strand sandwich assay for DNA detection. Intrinsic amplification of individual PNP probes should arise from the highly fluorescent terthiophene groups contained in the core of the particles.

 

Multicompartment Micelles: Higher Order Self-Assembly of Block Copolymers
6:00 PM-8:00 PM, Tuesday, 12 September 2006 Moscone Center -- Hall D, Poster

Division of Polymer Chemistry

The 232nd ACS National Meeting, San Francisco, CA, September 10-14, 2006