Signal amplifying DNA microarray by means of a novel conjugated polymer

POLY 75

Kangwon Lee, kangwonl@umich.edu1, Jean-Marie Rouillard, jmrouill@umich.edu2, Jae Cheol Cho1, Erdogan Gulari2, and Jinsang Kim1. (1) Department of Materials Science and Engineering, University of Michigan, Ann Arbor, MI 48109, (2) Department of Chemical Engineering, Materials Science and Engineering, and Macromolecular Science and Engineering, University of Michigan, 2300 Hayward. St., 3074 H.H.Dow, Ann Arbor, MI 48109
We report a newly developed conjugated polymer having unique stability in harsh conditions and its application for signal amplifying DNA chips. The unique stability of the new conjugated polymer allows a fast and convenient on-chip DNA synthesis directly on the new conjugated polymers. The new poly(oxadiazole-phenylene-co-fluorene) having amine side chains were designed, synthesized, and immobilized on the glass substrate. After immobilization of polymer by covalent bonding, oligonucleotides were directly synthesized on the emissive polymer. During the harsh DNA synthesis steps Polymer's emissive property maintained. In hybridization tests with a dye-labeled target DNA (HEX dye), we observed a large signal amplification of HEX fluorescence by means of efficient Förster energy transfer (FRET) from polymer to dye.
 

Polymers in Biosensors and Biochips
1:30 PM-4:40 PM, Sunday, 10 September 2006 San Francisco Marriott -- Salon 12/13, Oral

Division of Polymer Chemistry

The 232nd ACS National Meeting, San Francisco, CA, September 10-14, 2006