Antibody microarray using molecular-shape imprinting on a polymer containing azo dye

POLY 76

Fumihiko Hoshino, hoshino77@mosk.tytlabs.co.jp1, Taiji Ikawa1, Makoto Mouri1, Mamiko Narita1, Osamu Watanabe, e0909@mosk.tytlabs.co.jp1, Yuki Ueno2, Takanori Tsuda3, and Toshihiko Osawa, osawat@agr.nagoya-u.ac.jp4. (1) Watanabe Research Group, Toyota Central Research & Development Labs., Inc, Nagakute, Aichi, Japan, (2) Research Center for Biomarkers of Preventive Medicine, Doshisha Univ, (3) Res. Ins. for Biol. Functions, Chubu Univ, (4) Graduate School of Bioagricultural Science, Nagoya University, Chikusa, Nagoya, 464-8601, Japan
The genomics revolution has brought us DNA microarrays, however has encountered significant difficulties in realizing protein arrays. We developed a simple technique for nanostructure formation on an azo-benzene containing polymer (azopolymer), and have created a novel and a simple methodology for biomolecules immobilization on the surface of an azopolymer. The immobilization on the surface of the azopolymer film was accomplished by means of photoirradiation, inducing photoisomerization of azo moiety and then resulting in deformation of the film surface along the biomolecules. Activities of the biomolecules, such as enzyme reaction, antigen-antibody reaction were observed on the surface. We have applied this technique for protein microarrays in the format of microscopic glass slides. Antibodies are immobilized on glass slides on which the azopolymer is coated with 40nm-thickness (azoslide). We demonstrate a detection method of sandwich immunoassay, and clarify the correlation with conventional ELISA for detection of mouse Acrp30 in culture supernatant.
 

Polymers in Biosensors and Biochips
1:30 PM-4:40 PM, Sunday, 10 September 2006 San Francisco Marriott -- Salon 12/13, Oral

Division of Polymer Chemistry

The 232nd ACS National Meeting, San Francisco, CA, September 10-14, 2006