Preparation of bioconjugated hydrogels that respond to target biomolecules

POLY 278

Takashi Miyata, tmiyata@ipcku.kansai-u.ac.jp, Kaori Okawa, Masashi Jige, Chihiro Ohba, and Tadashi Uragami, uragami@ipcku.kansai-u.ac.jp. Faculty of Engineering, Kansai University, 3-3-35, Yamate-cho, Suita, Osaka, 564-8680, Japan
We have prepared two types of biomolecule-responsive hydrogels (biomolecule-cross-linked hydrogel and biomolecule-imprinted hydrogel) by using biomolecular complexes as reversible cross-linking points. This paper describes two biomolecule-responsive hydrogels that undergo volume changes in response to target biomolecules such as tumor marker glycoprotein and single-stranded (ss)DNA, which were prepared by using biomolecular complexes such as antigen-antibody complexes, saccharide-lectin complexes and DNA duplexes. DNA-responsive hydrogels as biomolecule-cross-linked hydrogels and tumor marker-responsive hydrogels as biomolecule-imprinted hydrogels were prepared by bioconjugation of PAAm networks with DNAs, lectins and antibodies. DNA-cross-linked hydrogels recognized the sequence of ssDNA and swelled gradually in response to the sequence of ssDNA. Tumor marker-imprinted hydrogels only shrank when both lectin and antibody in the hydrogels simultaneously recognized the saccharide and peptide chains of the target glycoprotein.