CELL 26 |
| Oxidation reactions are usually monitored by liquid chromatography (coupled with spectrophotometry or mass spectrometry detection). However, this technique only provides information on the disappearance of the reactant and the formation of the lower molecular weight oligomers. Due to the lack of separation, higher oligomers and polymers cannot be analysed. An alternative approach based on mono and bi-dimensional NMR techniques was developed to monitor oxidation kinetics and identify the reaction products. Catechin and caffeic acid have been selected as the most representative catechol systems involved in oxidation processes. The difficulty linked to the analysis of the raw reaction mixture results from the structural similarity between oxidation products and their precursor. This is overcome by 2D-DOSY experiments which indicate the extent of oxidative polymerisation by measuring the diffusion coefficients of the molecules in solution. Therefore, this technique appears promising to develop fingerprint analysis of oxidation processes and profiling of biological fluids. |
|
Poster Session
4:00 PM-6:00 PM, Sunday, 10 September 2006 Moscone Center -- Hall D, Poster
Division of Cellulose & Renewable Materials |