Structure-function characterization of photopolymerized dimethacrylate scaffolds

POLY 371

Sheng Lin-Gibson,, Forrest A. Landis,, James A. Cooper, Jean S. Stephens,, and Marcus T. Cicerone, Polymers Division, NIST, 100 Bureau Drive, Stop 8543, Gaithersburg, MD 20899
Model tissue engineering scaffolds based on a photopolymerized dimethacrylate resin were prepared for various structure-function characterizations, including scaffold structure, mechanical properties, and optical imaging of cell attachment. Scanning electron microscopy and X-ray microcomputed tomography confirmed that the scaffold structure could be controlled by the size distribution of NaCl porogen. The mechanical properties such as modulus increased as the porogen size increased. The scaffolds were also seeded with osteoblasts, cultured between 3 d to 18 d, and examined using confocal microscopy. Optical images of cells could be obtained at over 400 micron beneath the surface of the scaffold due to the amorphous glassy nature of the polymer.