Apatite with low crystallinity is a superior carrier for macromolecular drug delivery to mammalian cells

ANYL 154

Ezharul Hoque Chowdhury, ezhar888@yahoo.com, Biomolecular Engineeering, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, 226-8501, Japan
Two unique properties of carbonate apatite which are well-known in hard tissue development, have never been disclosed in gene delivery technology. We report here on the development of an efficient gene delivery system based on those properties of generated carbonate apatite - ability of preventing the growth of crystals needed for efficient cellular uptake of DNA and the high dissolution rate due to the low crystallinity of the crystals, for rapid intracellular release of DNA. While comparing with fluoridated carbonate apatite and hydroxyapatite which lackone or both of the aforementioned properties, carbonate apatite shows remarkably higher transfection efficiency. Considering its efficiency level in gene transfer, safety and simplicity, this newly developed technique is highly promising over other existing ones for gene therapy applications.
 

General Papers
7:00 PM-9:00 PM, Sunday, 10 September 2006 Moscone Center -- Hall D, Poster

Division of Analytical Chemistry

The 232nd ACS National Meeting, San Francisco, CA, September 10-14, 2006