Polymer waveguides integrated to microfluidics for reading microarray hybridization events

ANYL 260

Feng Xu, fengxu@lsu.edu1, Hong Wang1, Proyaq Datta2, Suying Wei1, Sitanshu Gurung2, Jost Goettert2, and Stephen A. Soper, chsope@lsu.edu3. (1) Department of Chemistry, CBM2, Louisiana State University, Baton Rouge, LA 70803, (2) CAMD, Louisiana State University, (3) Louisiana State University, Baton Rouge, LA 70803
Microarrays offer the ability to screen a large panel of biomarkers in parallel and when coupled to fluorescence, offers low detection limits. However, the readout of arrays is typically accomplished in a serial fashion using scanning-type confocal imagers. In this work, we describe a highly parallel readout format, which is based upon polymer waveguides that also serve as the immobilization support for the probes. We report on the coupling of polymer waveguides for reading hybridization events to microfluidics, which provide the ability to prepare the samples prior to incubation with the arrayed probes. The waveguide is fabricated through a double-sided hot-embossing technique to create the air-embedded waveguide. Oligonucleotide probes are covalently strapped to the waveguide following UV-activation of the polymer surface. Low-abundant BRCA1 mutations were successfully detected.
 

Interfacing Biology with Lab-On-A-Chip Separations
8:30 AM-11:55 AM, Wednesday, 13 September 2006 Moscone Center -- Room 130, Oral

Division of Analytical Chemistry

The 232nd ACS National Meeting, San Francisco, CA, September 10-14, 2006