Operation of biosensors in nonaqueous solvents: Effect of the solvent

ANYL 299

Niall J. O'Reilly, niall.oreilly@ul.ie, Zuzana Brusova, Anastassija Konash, Ekaterina Ivanova, Suzanne Crilly, and Edmond Magner, edmond.magner@ul.ie. Materials & Surface Science Institute, University of Limerick, Limerick, Ireland
The catalytic properties of peroxidase from horseradish (HRP) were studied in organic media. Peroxidase was immobilized onto glassy carbon electrode by entrapment in Eastman AQ 55. Ferrocene dimethanol was used to mediate electron transfer between the protein and the electrode. Using cyclic voltammetry and chronoamperometry the dependence of the catalytic current on the concentration of hydrogen peroxide and of ferrocene dimethanol was examined in acetonitrile, acetone, ethanol, methanol, 1-propanol, 2-propanol, 1-butanol, 1,2-propanediol, all of them contained 5% of water.