Apparatus and results for pressurized planar electrochromatography (PPEC)

ANYL 305

A. L. Novotny, allnovot@iupui.edu1, D. Nurok, nurok@chem.iupui.edu1, R. E. Santini, rsantini@purdue.edu2, R. W. Replogle2, and G. L. Hawkins2. (1) Department of Chemistry, IUPUI, 402 N Blackford St, Bldg LD Rm 325, Indianapolis, IN 46202, (2) Department of Chemistry, The Amy Facility for Chemical Instrumentation, Purdue University, 560 Oval Drive, West Lafayette, IN 47907
Pressurized Planar Electrochromatography (PPEC) is a new separation technique that is both substantially faster and more efficient than classical Thin Layer Chromatography (TLC). The mobile phase is driven by electroosmotic flow, while the sorbent layer is pressurized in such a manner that the separation temperature is controlled. PPEC retains the attractive features of TLC, and this includes the ability to simultaneously separate multiple samples. For this reason the technique is very suitable for high-throughput analyses.

Our presentation will discuss the apparatus for PPEC, and the dependence of separation quality on variables that include the applied electric field, the applied pressure, the temperature of separation, the buffer concentration and the nature of the sorbent layer. Separations of different classes of compounds will be discussed. The separation of nine compounds in two minutes illustrates the speed of analysis. Under optimum conditions an extrapolated efficiency of over 100,000 plates per meter is obtained.

 

Analytical Approaches: Separation Science
8:30 AM-12:00 PM, Thursday, 14 September 2006 Moscone Center -- Room 130, Oral

Division of Analytical Chemistry

The 232nd ACS National Meeting, San Francisco, CA, September 10-14, 2006