ANYL 287 |
| Analysis of complex mixtures by electrospray ionization mass spectrometry is compromised by ionization suppression, a phenomenon in which certain analytes are preferentially ionized at the expense of others. This problem has led to the development of complex, expensive and time-consuming separation schemes for proteomic and metabolomic analyses. We propose to address ionization suppression in a different manner. A single, charged, parent droplet will be trapped in front of the mass spectrometer inlet using a single-axis acoustic levitator. The parent droplet will be allowed to desolvate until it reaches the Rayleigh stability limit, at which time it will electrospray charged progeny droplets, and these smaller droplets will be directed into the mass spectrometer by an electric field. The parent droplet remains trapped in the levitator, and it will be recharged by using a corona discharge or by adding charged solvent droplets with a piezoelectric dispenser. We anticipate repeating this process of desolvation, electrospray, and recharging multiple times in order to ionize analytes that normally remain uncharged in parent electrospray droplets. |
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Analytical Approaches: Mass Spectrometry
1:30 PM-4:20 PM, Wednesday, 13 September 2006 Moscone Center -- Room 124, Oral
Division of Analytical Chemistry |