Microwave assisted extraction of hydroxylated compounds in human serum

ANYL 346

Rebecca Heltsley, Rebecca.Heltsley@nist.gov, Hollings Marine Laboratory, National Institute of Standards and Technology, 331 Fort Johnson Rd, Charleston, SC 29412, John R. Kucklick, john.kucklick@nist.gov, Hollings Marine Laboratory, NIST, 219 Ft. Johnson Road, Charleston, SC 29412, and Michele M. Schantz, michele.schantz@nist.gov, Analytical Chemistry Division, National Institute of Standards and Technology, 100 Bureau Dr, Stop 8392, Gaithersburg, MD 20899.
Recently, endocrine-disrupting compounds, such as phenolic halogenated compounds and hydroxylated PCBs, have gained widespread interest due to their potential health effects in humans and wildlife. The two primary matrices used to assess human exposure to environmental contaminants are urine and blood. Blood serves as a circulating reservoir for phenolic and hyroxylated compounds due to their capacity to bind to proteins. Simple, efficient extraction and analytical techniques for these compounds in blood are a priority. This study will provide an alternative method for extracting phenolic and neutral compounds from blood using open-focused microwave technology. The application of a semi-automated microwave extraction technique reduces the time required for sample preparation and provides a relatively high throughput method compared to existing labor intensive liquid-liquid extraction methodology. Method development was performed on two candidate reference materials, SRM 1957 Organic Contaminants in Human Serum (non-fortified) and SRM 1958 Organic Contaminants in Human Serum (fortified). Values generated and certified in these materials will ultimately provide researchers with reference materials to use when extracting hydroxylated analytes in blood.
 

Analytical Approaches: Various Techniques and Applications
1:30 PM-4:00 PM, Thursday, 14 September 2006 Moscone Center -- Room 124, Oral

Division of Analytical Chemistry

The 232nd ACS National Meeting, San Francisco, CA, September 10-14, 2006