Bacteriophage amplification with immunochromatographic detection of Yersinia pestis

ANYL 259

Kent J. Voorhees, kvoorhee@mines.edu1, Leah G. Luna, ldoan@mines.edu1, and Scott W. Bearden, zyv3@cd.gov2. (1) Department of Chemistry, Colorado School of Mines, 1500 Illinois Street, Golden, CO 80401, (2) Bacterial Zoonoses Branch/ Diagnostic and Reference Laboratory, Centers for Disease Control and Prevention Division of Vector-Borne Infectious Diseases, PO Box 2087, Foothills Campus, Fort Collins, CO 80522
Bacteriophage amplification with immunochromatographic strips (BAmICS) is a new technology under development for rapid detection in disease diagnostics. BAmICS takes advantage of phage specificity for a bacterial pathogen and progeny production for amplification. Detection is achieved with reporter conjugated phage antibodies on an immunochromatographic strips (ICS). When progeny phage bind to these antibodies, the accumulation of the reporter molecule must exceed a predetermined threshold concentration in the capture zone in order to be detected. When the pathogen of interest is present, progeny phage replication ensues, binds to the conjugated antibodies on the ICS resulting in a reporter concentration that increases above the threshold in the capture zone providing a detectable signal. If the targeted pathogen is not present, the threshold will not be reached, resulting in a non-detectable signal. This presentation discusses the development and characterization of BAmICS for the detection of Yersinia pestis, the etiological agent of the plague.
 

Disease Diagnostics
8:30 AM-10:55 AM, Monday, 27 March 2006 Georgia World Congress Center -- B214, Oral

Division of Analytical Chemistry

The 231st ACS National Meeting, Atlanta, GA, March 26-30, 2006