ANYL 90 |
| The need to analyze and identify low protein copies requires high sensitivity and throughput technologies to address sample complexity and limited volumes. Coupling microfluidics to biological MS is a logical step towards developing such capabilities. we are developing a micrototal analysis system for sample processing before MS readout. We will report on an integrated polymeric microfluidic network for a bottom up proteomics strategy. The device is microfabricated by micromilling and hot embossing technologies and assembled by thermally annealing. The microfluidic network incorporates a two dimensional separation and an enzymatic solid phase micro-reactor is integrated to the 2D separation system to digest protein bands eluted from the second dimension, before further downstream separation of the generated peptide fragments using capillary electrochromatography (CEC). Peptide mass maps are acquired online using a linear matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) coupled to the microdevice using a rotating ball inlet interface.
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General Session
7:00 PM-9:00 PM, Sunday, 26 March 2006 Georgia World Congress Center -- Ex. Hall B4, Poster
Sci-Mix
Division of Analytical Chemistry |