Effect of pH on genetically engineered glucose fluorescence nanosensors (GFN)

ANYL 268

Xinxin Wu, xxw02@uark.edu1, Sha Jin, sjin@uark.edu2, and Kaiming Ye, kye@uark.edu1. (1) Department of Biomedical Engineering, University of Arkansas, 700 Research Center Blvd., Room 3420, Fayetteville, AR 72701, (2) DNA and Protein Core Facility, University of Arkansas, Fayetteville, AR 72701
We have engineered a fluorescence nanosensor for continuous glucose monitoring. The sensor is constructed by direct introducing a FRET signal transduction function into a mutant glucose binding protein (GBP) so that the conformation changes in the spatial structure of GBP upon glucose binding generate a FRET signal between two fluorescent proteins that genetically fused to two ends of GBP. We have demonstrated that this sensor has a good response to glucose until 1.2 mM. To adopt this sensor for determining the glucose concentrations within living cells, we investigated pH effect on the dynamic behavior of the sensor. By fusing different GFP variants such as a pH insensitive CFP and YFP, we studied the pH effect on the FRET efficiency upon glucose binding. The results will be reported in details at the conference.
 

Analytical Approaches
8:30 AM-12:00 PM, Monday, 27 March 2006 Georgia World Congress Center -- B215, Oral

Division of Analytical Chemistry

The 231st ACS National Meeting, Atlanta, GA, March 26-30, 2006