Metal particle enhanced fluorescent immunoassays on metal mirrors

ANYL 110

Evgenia G. Matveeva, evgenia_matveeva@hotmail.com, Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, 108 N. Greene Street, Baltimore, MD 21201, Ignacy Gryczynski, Department of Cell Biology and Genetics, University of North Texas, Health Science Center, 3500 Camp Bowie Blvd., Fort Worth, TX 76107, Joseph R Lakowicz, Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland at Baltimore, 725 W. Lombard St., Baltimore, MD 21201, and Zygmunt Gryczynski, zgryczyn@hsc.unt.edu, Department of Molecular Biology and Immunology, University of North Texas, Health Science Center, 3500 Camp Bowie Blvd., Fort Worth, TX 76107.
We present fluoroimmunoassays on plain metal coated surfaces (metal mirrors) enhanced by metal nanoparticles (silver island films, SIFs). Continuous metal layer (aluminum, gold, or silver protected with a thin silica layer) were coated with SIFs, and an immunoassay (model assay for rabbit IgG, or myoglobin immunoassay) was performed on this surface using fluorescently labeled antibodies. Our results showed that SIFs alone (on glass surface not coated with metal) enhance the immunoassay signal approximately 3 to 10-fold. Using a thin metal layer deposited on glass as support for SIFs leads to up to 50-fold signal enhancement.

 

General Session
7:00 PM-9:00 PM, Sunday, 26 March 2006 Georgia World Congress Center -- Ex. Hall B4, Poster

Sci-Mix
8:00 PM-10:00 PM, Monday, 27 March 2006 Georgia World Congress Center -- Ex. Hall B4, Sci-Mix

Division of Analytical Chemistry

The 231st ACS National Meeting, Atlanta, GA, March 26-30, 2006