Resistive pulse sensing of proteins using single conical nanopores in PET membranes

ANYL 147

Lindsay T. Sexton, ltaylor@chem.ufl.edu1, Pu Jin1, Kaan Kececi2, Lane Baker1, Youngseon Choi1, and Charles R. Martin1. (1) Department of Chemistry, Center for Research at the Bio/Nano Interface, University of Florida, PO Box 117200, Gainesville, FL 32611, (2) Center for Research at the Bio/Nano Interface, Department of Chemistry, University of Florida, Gainesville, FL 32611-7200
Resistive pulse sensors have been used to detect and analyze a variety of materials ranging from nanometer sized colloidal particles, viruses, DNA, polymers, metal ions, small organic molecules, and proteins. In this work a single conical gold nanopore functionalized with polyethylene glycol (PEG) thiol has been utilized as the sensing element for the detection and characterization of proteins and protein-ligand pairs. The conical nanopores were prepared in a polyethylene terephthalate (PET) membrane by the track-etch method and subsequently electolessly plated with Au. The nanopore was functionalized with PEG-thiol to prevent non-specific protein absorption onto the pore walls. The goal of this study is to determine if the current profiles of proteins change in the presence of a binding ligand. We will present our initial studies of the time-dependent ionic current through these conical nanopores in response to analyte proteins present in solution.
 

General Session
7:00 PM-9:00 PM, Sunday, 26 March 2006 Georgia World Congress Center -- Ex. Hall B4, Poster

Sci-Mix
8:00 PM-10:00 PM, Monday, 27 March 2006 Georgia World Congress Center -- Ex. Hall B4, Sci-Mix

Division of Analytical Chemistry

The 231st ACS National Meeting, Atlanta, GA, March 26-30, 2006