ANYL 81 |
| Ribosomes, the protein workshop of the cell, are responsible for the production of proteins in all living organisms. The ribosome is a ribonucleoprotein (RNP) complex composed of both ribosomal proteins and ribosomal RNA. The exact role of ribosomal proteins, other than simply providing structural scaffolding, is not presently known. A fundamental prerequisite for understanding the biochemical interactions that occur within the ribosome during protein synthesis is a detailed knowledge of the structure of the ribosome. Matrix-assisted laser desorption/ionization time-of flight mass spectrometry (MALDI-TOF MS) is becoming a valuable tool for probing the structure of the ribosome. This work presents several applications of this tool, including the development of a method to evaluate and compare mixtures of ribosomal proteins as well as a method for the employment of MALDI-TOFMS combined with the traditional biochemical technique of limited proteolysis for evaluating the ribonucleoprotein complex |
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General Session
7:00 PM-9:00 PM, Sunday, 26 March 2006 Georgia World Congress Center -- Ex. Hall B4, Poster
Sci-Mix
Division of Analytical Chemistry |