FUEL 25 |
| Immobilizing cytochrome c oxidase and cytochrome c/ cytochrome c oxidase complex into lipid bilayer membranes on the gold electrodes were investigated. The direct electron transfer for electroreduction of oxygen from electrode substrate to enzyme was observed on both kinds of the modified electrodes. Cytochrome c/cytochrome c oxidase complex promoted significant electron transfer rate of reduction oxygen. The pH, component, ionic strength of solution and temperature also affect the electron transfer between enzyme and electrode substrate. Although the direct electron transfer between enzyme and electrode take place on both kinds of the modified electrodes, the electrocatalyzed reduction of oxygen is controlled by electron transfer. Slow electron transfer is due to non-controlled immobilization, which traps the metalloprotein in non-optimal orientations. If the assembled protein complex to the electrode is optimized, the cytochrome c/ cytochrome c oxidase complex electrode has the potential use for the direct electroreduction of oxygen to water in the cathode compartment of a biofuel cell. |
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Biofuels for Transportation
1:30 PM-4:45 PM, Sunday, 26 March 2006 Georgia World Congress Center -- C204, Oral
Division of Fuel Chemistry |