Separation of fatty acids by non-aqeous capillary electrophoresis

CHED 234

Candace H. Payne, candhan@yahoo.com1, John T. Williams, william800@duq.edu1, David L. Gallaher Jr., gallaherdl@carlow.edu2, and Mitchell E. Johnson, johnsonm@duq.edu1. (1) Department of Chemistry and Biochemistry, Duquesne University, Mellon Hall, Pittsburgh, PA 15282, (2) Department of Chemistry and Physics, Carlow University, 304 A.J. Palumbo Hall of Science and Technology, 3333 Fifth Avenue, Pittsburgh, PA 15213
Within recent years, Capillary Electrophoresis (CE) has become extremely popular in the separation of biological sample analytes such as fatty acids. Although CE allows for the use of minute volumes, which improves separations; the technique often has issues with adsorption of analytes to the capillary walls. The development of means to reduce this problem is fundamental. In this work, fatty acids were derivatized with a positively charged, near-infrared absorbing, fluorescent dye. To prevent adsorption, two approaches were used: one capillary was coated with trimethylsilane (TMS) with a positively charged surfactant added to the run buffer; while another with Successive Multiple Ionic Polymer Layers (SMIL). Separations were done using a similar series of saturated and unsaturated fatty acids. Adsorption was assessed by monitoring the quality of the separations.
 

Undergraduate Research Poster Session: Analytical Chemistry
11:00 AM-1:00 PM, Monday, 27 March 2006 Georgia World Congress Center -- Ex. Hall B4, Poster

Division of Chemical Education

The 231st ACS National Meeting, Atlanta, GA, March 26-30, 2006