ANYL 187 |
| SPR analysis is a powerful label-free biointeraction and sensing method, but is currently confined to the use of SAMs and dextran on gold. While these techniques are suitable for many applications, their limitations are also well known: pinholes, non-specific adsorption, instability, etc. We report a new alternative, nanoscale glassified silicate layers on gold, fabricated by spraying on alternate layers of a cationic polyelectrolyte and sodium silicate followed by calcination. Smooth silicate films were made on gold substrates with thicknesses from 2-50nm. The properties of the new glassified substrates were characterized using SEM, fluorescence, FRAP, and SPR methods. The substrates were smooth enough to support formation of supported bilayer membranes (SBMs) with diffusion on the large scale. Additional SEM characterization revealed the surface topology. The new surface is being employed in several projects that take advantage of the ability to use glass-based chemistry in conjunction with SPR analysis. The first project showed the formation of SBMs and use as a sensor for avidin and cholera toxin with high sensitivity and minimal non-specific adsorption, even at protein concentrations as high as 0.1 mg/mL. |
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General Session
7:00 PM-9:00 PM, Sunday, 26 March 2006 Georgia World Congress Center -- Ex. Hall B4, Poster
Sci-Mix
Division of Analytical Chemistry |