Simple method in diagnosing cancer cells by a novel fluorescence probe BMVC

ANYL 137

Ta-Chau Chang, tcchang@po.iams.sinica.edu.tw, Institute of Atomic and Molecular Sciences, Academia Sinica, P.O. Box 23-166, Taipei, 106, Taiwan, Cheng-Chung Chang, ccchang@pub.iams.sinica.edu.tw, Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei, 106, Taiwan, and Chi-Chih Kang, IAMS, Academia Sinica, Taipei, 106, Taiwan.
A number of carbazole derivatives (US patent 10/690,984) were designed and synthesized for distinguishing cancer cells from normal cells. The fluorescence images of different cancer cells and normal cells after treatment with 0.1 µM 3,6-bis(1-methyl-4-vinylpyridinium)carbazole diiodide (BMVC) were examined and presented. Over 70 % of the cells showed bright fluorescence spots in the nucleus of cancer cells, but less than 20 % of cells showed the bright spots in the nucleus of normal cells, suggesting that BMVC is a novel fluorescence marker for the application in the diagnosis of cancer cells. Moreover, different cellular accumulations of BMVC in cancer cells from normal cells allow us to establish a simple and economic method for the diagnosis of cancer cells.
 

General Papers
7:00 PM-9:00 PM, Sunday, 28 August 2005 Washington DC Convention Center -- Hall A, Poster

Division of Analytical Chemistry

The 230th ACS National Meeting, in Washington, DC, Aug 28-Sept 1, 2005